By reviewing the literature of KM, it can be understood that there are different views about(to) knowledge management and its applications in public and private sectors which is due to the structural difference between these two economic sectors. In order to be able to exploit the benefits of knowledge management in public sector adequately, it is necessary to identify the best practices of KM. To understand the differences between knowledge management in public and private sector and benchmark capabilities of private sector in public sector, is one of the ways to achieve this goal. In this study, we used survey study and questionnaire tool in order to identify these differences in banks. Results suggest that just in two dimensions of information technology and knowledge management, among five identified dimensions, there is a meaningful difference between public and private banks.