Nowadays it is very unlikely to come across an organization in which Information and Communication Technology is not discussed and hasn't become one of the apprehensions of the organizational managers.To insure an effective competitive edge in educational organizations, universities from all across the world must consistently improve their Information and Communication Technology.The purpose of this research was to investigate the amount of knowledge and use of Information and Communication Technology by employees. Information gathering was done by developing a questionnaire. The population of interest consisted of all the faculty members of the College of Agriculture and Natural Resources, University of Tehran, and a random group of 124 members were selected for statistical analysis. The validity of the questionnaire was approved by the statistics professionals and the foundation of it was approved by calculating the Cronbach’s alpha to 0/95.The results show that the degree of ICT use is directly and positively proportional to the educational degrees and academic ranking, and negatively proportional to work experience, and showed no correlation to age.The results of mean analysis showed a difference between the amount of usage of and familiarity with ICT between men and women and the variance analysis results showed that the amount of familiarity with and usage of ICT is different between different levels of education, academic ranks and different types of employment. Finally the results of regression analysis showed that the three variables of level of education, type of employment and age had a meaningful effect on the dependent variable of amount of familiarity with ICT, and the three variables of familiarity, type of employment and gender had a meaningful effect on the dependent variable of amount of usage of ICT. In general increasing the amount of familiarity with ICT is the most important factor effecting organization usage.